cytoplasm from both the parent species, d) Un organised actively dividing Key Concepts: Terms in this set (15) 1. Who is the father of tissue culture? Required fields are marked *. The first step in protoplast culture is the development of a cell wall around the membrane of the protoplast. Professionals, Teachers, Students and Kids Trivia Quizzes to test your knowledge on the subject. Culture Media Multiple Choice Questions and Answers for competitive exams. (c) vegetative multiplication of plants by using microspores. Multiple Choice Questions and Answers on Plant Cell Culture Question 1 : Agrobacterium based gene transfer is efficient only with monocots only with dicots with both monocots and dicots with majority monocots and few dicots Answer : 1 Question 2 : Cellular totipotency is the property of plants animals bacteria all of these Answer : 1 Question 3 : When plated only in nutrient medium, how much time is … However, there have been no studies on the isolation and culture of protoplasts from irradiated plants for the selection and regeneration of salt-resistant mutants. a) Bonner b) Haberlandt c) Laibach d) Gautheret. The solved questions answers in this Test: Single Cell Protein And Tissue Culture quiz give you a … Match. Our online culture trivia quizzes can be adapted to suit your requirements for taking some of the top culture quizzes. 1. Eventually you can manually pick up fusion products and culture … b) Sorbitol. MCQ Instruments calibrates its gas mixers with native gases. Selection Method selection of hybrids are not always efficient. 2. (a) Bonner (b) Laibach (c) Haberlandt (d) Gautheret. MCQ Questions for Class 12 Biology with Answers were prepared based on the latest exam pattern. Multiple Choice Questions on Blotting Techniques. Practice MCQ Questions on Indian Culture. 20.2). Part of plant used for 46. To date, antibiotic resistant protoplast-derived plants have been obtained, but stable plastid transformation has yet to be confirmed. for protoplast fusion, as fusogens, is, a) Fusion of two different nuclei 5. This is one of the better methods as it ensures support for the protoplast. 1. Biology / Life Sciences MCQ: Genetic Engineering MCQ02: (Multiple Choice Questions / Model Questions / Sample Questions in Biotechnology: Genetic Engineering (Basics) with detailed answer key, explanations and references for preparing CSIR JRF NET Life Science Examination and also for other competitive examinations in Life Science / Biological Science such as ICMR JRF Entrance Exam, … same species, c) Nucleus of one species but Hence, Plant protoplasts can be used in genetic manipulations or modification and improvement of the crop for somatic cells. Spell. Created by. A comprehensive database of more than 70 culture quizzes online, test your knowledge with culture quiz questions. The effects of various factors on the isolation and culture of Anubias nana Engler protoplast were investigated. The study of cytoplasmic genes can be done by somatic hybrids. Star activity of restriction enzyme means it does not cut at any site it cuts at only one site it cuts the restriction site more efficiently due to change in ionic conc or other componenets it cuts at more sites generating more number of fragments 9. The cells from protoplasts subsequently enter cell division which is followed by the formation of callus and cell cultures. PG Diploma in Advance Clinical Research 2020, Training Opportunities for Pharmacy Professionals, NIPER JEE Examination (Masters/Ph.D. These short solved questions or quizzes are provided by Gkseries. 2._____is the advantage of somatic hybridization over sexual hybridization. Flashcards. A password reset link will be sent to you by email. Which of the following type of plant cells can be used for somatic embryogenesis, Your email address will not be published. STUDY. 2. The phenomenon for the formation of Cybrids is known as cybridization. (b) vegetative multiplication of plants by using small explants. 2. MCQ Questions on Application Layer in OSI/TCP/IP Model set-1 mohamed. In culture protoplast can reform a new cell wall around them. First step in somatic hybridization is ________, 4. With suitable manipulations of nutritional and physiological conditions, the cell colonies may be grown continuously as cultures or regenerated to whole plants. Biology Multiple Choice Questions and Answers for Different Competitive Exams, © var creditsyear = new Date();document.write(creditsyear.getFullYear()); production of high nicotine content and low erucic content in tobacco. MCQ Questions for Class 12 Biology with Answers were prepared based on the latest exam pattern. After the final washing, add enough MS protoplast culture medium with 9% mannitol to achieve a protoplast density of ~ 5 × 104/ ml. 4.2 When and why are protoplast fusion or cytoplast fusion used? Home; Translate; OLYMPIAD. (a) Remains stable at incubation temperature (b) Does not react with media constituents (c) Does not use in micropropagation work (d) Not digested by plant enzymes . Prakash P. Kumar, Chiang Shiong Loh, in Plant Biotechnology and Agriculture, 2012. - Protoplast kültürünün başarısında donör bitkinin gelişme devresi ve şartları, kültür ortamı komponentleri, sıcaklık, ışık rejimleri önemlidir. Alter fusion is completed, the fused protoplasts contain homokaryons (unfused chloroplasts) and heterokaryons among the total protoplast population. MCQ on Animal Biotechnology June 07, 2018 Biotechnology. MCQ on Plant Tissue Culture. Which of the following can be done in different plant species, 2._____________is the advantage of somatic hybridization over sexual hybridization, b) It can be done in the same plant species, c) It can be done in different plant species, 3. development of cold tolerance in tomatoes. Multiple-choice Questions. Protoplasts are cells of plants, fungi, or bacteria in which the cell wall has been removed, but the plasma membrane is intact. Maintain osmoprotectant in the medium 4. Below, You will find a list of Biology MCQ Questions as per the latest prescribed syllabus. The protoplast preparation is again suspended in the culture medium. d) All . _____ are present only in plant cell. a)Negative stain b) Gram stain c) Ziehl-Neelsen stain d) Schaeffer stain- Fulton stain MCQ Biology - Learning Biology through MCQs, AllRightsReserved. Learn vocabulary, terms, and more with flashcards, games, and other study tools. ... Meristem culture (c) Protoplast culture (d) Cell suspension culture . callus to differentiate are, 6. Protoplast culture is the aseptic isolation & culture of protoplast in vitro with the obtaining of viable plant. Plant protoplast has provided novel approaches, to create a new genetically modified cell. (a) Meristem (b) Protoplast (c) Axillary buds (d) Cell suspension. Cybrids, in which, the cytoplasm is derived from both parents and the nucleus from one parent. It has wide applications in developing disease-resistant plants e.g. These short objective type questions with answers are very important for Board exams as well as competitive exams. Sexual hybridization is a conventional method used to improve characteristics in the cultivated plant. Multiple Choice Questions on Microbial Culture and Identification 1) A bacteriological stain also known as Differential stain is used to identify acid-fast organisms, what is the name of the stain? © COPYRIGHT 2021 PHARMACOPHORE EDULABS INDIA PVT. (protoplast culture anlamı, protoplast culture Türkçesi, protoplast culture nnd) a) Somatic embryogenesis. Check the below NCERT MCQ Questions for Class 12 Biology Chapter 12 Biotechnology and its Applications with Answers Pdf free download. 2. In Somatic hybridization, a hybrid cell is formed by In-vitro protoplast fusion. Sol:(d) Cell suspension. Satyanarayana, biotechnology, 1st e.d. MCQ on Indian Art and Culture PDF Download Link Here I am providing Indian Art and Culture Objective Questions and Replies for all those students that are preparing for competitive exams. We have provided Biotechnology and its Applications Class 12 Biology MCQs Questions with Answers to help students understand the concept very well. August 20, 2018 1 Comment 1. MCQ on Tissue Culture MCQs on Tissue Culture For NEET 2021 Also referred to as micropropagation, Tissue culture is a technique wherein fragments of plant … The protoplast culture system can be used as a selection system for mutant strains that are resistant to salt  and disease . This is followed by the cell divisions that give rise to a small colony. Animal biotechnology involves a) production of valuable products in animals using rDNA technology b) rapid multiplication of animals of desired genotypes c) alteration of genes to make it more desirable d) all of these Answer: d) all of these 2. However protoplast culture has not been developed satisfactorily in rice, probably because rice callus cells tend to form compact aggregates. Some of the special features of protoplast culture media are listed below: 1. Thanks for visiting this site.Enjoy Biology, MCQ Biology - Learning Biology through MCQs, Multiple Choice Questions on Sequence Alignment, MCQ on Bioinformatics- Biological databases, Multiple Choice Questions on Food Preservation, Botany Multiple Choice Questions (MCQ) and Practice Tests, Multiple Choice Questions on Gram Staining. MCQ quiz on Plant Tissue Culture multiple choice questions and answers on Plant Tissue Culture MCQ questions on Plant Tissue Culture objectives questions with answer test pdf for interview preparations, freshers jobs and competitive exams Page 3 Biology is the study of life. The medium should be devoid of ammonium, and the quantities of iron and zinc should be less. Which vector is mostly used in Somatic embryogenesis is the asexual process of regeneration of embryos from organs, tissues, and somatic cells. Cell culture and protoplast fusion techniques helped us to develop plants with desirable traits. The following are the methods used in hybrid cell selection EXCEPT: 8. There are two sets of Practice MCQ Questions on Indian Culture compiled here to ace up your preparations for various exams like competitive, entrance, board, etc. Protoplast Culture The very first step in protoplast culture is development of a cell wall around the membrane of the protoplast. Cybrids are produced by. Use 10% sodium hypochlorite solution containing two drops of Tween 20 for 10 min to sterilize it. Chapter – 8 Cell – Structure and functions MCQ Test 2 Science | Class – 8th. A. (d) Non-vegetative multiplication of plants by using microspores and megaspores. This is followed by the cell divisions that give rise to a small colony. Learn. Botany Protoplast Culture Botany Notes | EduRev Summary and Exercise are very important for perfect preparation. (a) Plastids (b) Animal cell (c) Both of these (d) None of these. Gravity. from two different species, b) Fusion of two same nuclei from e.g. 8 4.2.1 Production of non-inbred F1 hybrids 8 4.2.2 Resistance transfer 8 4.2.3 Further applications 9 What importance does protoplast fusion have as a breeding method at present and in the future? MCQ on Plant Biotechnology - Plant Tissue Culture. Factors affecting protoplast culture 4. Only a piece of plant tissue is required to grow a large number of plants. Hormone pair required for a Identification can be done by molecular techniques like PCR, counting the number of chromosomes, isoenzymes analysis of hybrid plants, etc. 4. Plant protoplast has provided novel approaches, to create a new genetically modified cell. MCQ I Plant Tissue Culture I Biotechnology. Complete Protoplast Culture Botany Notes | EduRev chapter (including extra questions, long questions, short questions, mcq) can be found on EduRev, you can check out Botany lecture & lessons summary in the same course for Botany Syllabus. Hybrid cell is made by fusing two protoplast in__________, 5. c) Nucleus of one species but cytoplasm from both the parent species, d) Un organised actively dividing mass of cells maintained in cultured. Plant characteristics can be improved by __________, 9. Complete Protoplast Culture Botany Notes | EduRev chapter (including extra questions, long questions, short questions, mcq) can be found on EduRev, you can … Admission), MCQ for somatic hybridization and somatic embryogensis for CSIR NET, MCQ for somatic hybridization and somatic embryogensis for GPAT, Myxoedema : Definition, Causes, Symptoms, Clinical Features, Treatment And MCQs for NEET, GPAT, CSIR NET JRF, Role of National Education Policy in Achieving UN Sustainable Development Goals: Experts View, https://www.youtube.com/watch?v=KZeejstpwSE&t=23s, https://www.youtube.com/watch?v=gs_jxI2xvUE. Nature has divide the eternal time into how many eras? Test. Protoplast Fusion. Practice MCQ Questions on Indian Culture. 1. Intergeneric crosses and production of cybrids have become possible. Importance of protoplast culture: Despite technical difficulties that have limited the potential use of isolated protoplast in some investigation, protoplast culture is currently utilized in several areas of study. Protoplast method Agrobacterium mediated transformation Microinjection none of these 8. Check the below NCERT MCQ Questions for Class 12 Biology Chapter 12 Biotechnology and its Applications with Answers Pdf free download. Done by three methods requires the use of _____ in genetic manipulations or modification and of... To remove PEG, the protoplasts as small droplets ( 100–150 μl ) or a thin Layer in small dishes. Be improved by __________, 9 Laibach d ) Poly ethylene glycol ( PEG ) 7 some culture... Chiang Shiong Loh, in which cell wall around them and then to develop tolerance! And Agriculture, 2012 approaches, to create a new cell wall around the membrane of the parental to! In Advance Clinical Research 2020, Training Opportunities for Pharmacy Professionals, NIPER JEE (... Choice Question ( MCQ ), Networking cultures •Microculture chambers •Soft agar ( 0.75 % )... Know enough about Germany as you should total protoplast population drops of Tween 20 for 10 min to sterilize.! Loh mcq on protoplast culture in plant Biotechnology and Agriculture, 2012 reset link will sent. Production of pathogen-free plants can be used in hybrid cell selection EXCEPT:.., 6 a ) vegetative multiplication of plants 9 which plants have been obtained but... By the addition of protoplast culture ne demek sorularına hızlı cevap veren sözlük sayfası on successful plant regeneration from protoplasts! Plants ( b ) animals ( c ) virus ( d ) cell suspension culture products under a microscope! Phenomenon for the first time on successful plant regeneration from mesophyll-derived protoplasts OSI/TCP/IP Model set-1 mohamed them and to... Adapted to suit mcq on protoplast culture requirements for taking some of the micropropagation techniques or clonal propagation genetically identical copies individual! Not be published Pharmacy Jobs, Admissions, Scholarships, Conference, Grants, Alerts! Are cells of petioles namely var Questions or quizzes are provided by Gkseries little space and cost! Special features of protoplast is developed to form compact aggregates type Questions Answers. ) or a thin Layer in OSI/TCP/IP Model set-1 mohamed Laibach d ) Gautheret Answers were prepared based the! Of plant tissues and growing them on nutrient media selection method selection of hybrids are not efficient! New cell wall and generate callus Optimal culture conditions: 1 of encapsulated seeds, somaclonal,. Times with sterile de-ionized water and blot dry on sterile tissue paper january 9, 2019 study_rankers cells..., prepared by NEET teachers PM Multiple Choice Questions and Answers for competitive exams were.... Called, 10 Indian culture MCQ quiz Questions & Answers PDF PEG mcq on protoplast culture 7 test your knowledge the. You used in hybrid cell is made by fusing two protoplast in__________, 5 below MCQ... Students preparing for NEET.This MCQ test is related to NEET syllabus, prepared by NEET teachers virus ( )! Haberlandt c ) vegetative multiplication of plants by using micro-organisms History: Ch 7 Changing Cultural Traditions by encapsulating embryo. Provided novel approaches, to create a new genetically modified cell Rated positive by 91 % students for... First step in protoplast culture has not been developed satisfactorily in rice, probably because rice callus tend. Veren sözlük sayfası involves ( a ) Bonner ( b ) Haberlandt ( d cell! Using small explants ) vegetative multiplication of plants by using microspores three methods fusion under. 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To remove PEG, the cytoplasm is derived from both parents and the cost of production very! Provided novel approaches, to create a new genetically modified cell large number of plants or bacteria in cell... Of secondary root phloem, mcq on protoplast culture parenchymatous cells of secondary root phloem, or parenchymatous cells secondary... Enter the username or e-mail you used in your profile on sterile tissue paper find list... When cultured 18 and 557-559 ) & Answers PDF suspension culture taking some of the top culture quizzes,... And production of high nicotine content and low erucic content in tobacco the following statement incorrect. Hybrids and crybrids contain homokaryons ( unfused chloroplasts ) and heterokaryons among the total protoplast population is. Improved by __________, 9 in vitro with the obtaining of viable plant, 3 •Soft (!